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KMID : 0604520140400040391
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Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 4 p.391 ~ p.402
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Antioxidative Effects and Component Analysis of Extracts of the Rumex acetosa L.
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Jeong Yoo-Min
Kim Ho-Jae
Lee Su-Hyun
Jang Do-Yun
Choi Yae-Chan
Min Na-Young
Kong Bong-Ju
Park Soo-Nam
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Abstract
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In this study, the antioxidative effects and component analysis for the extracts of Rumex acetosa L. were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried R. acetosa L.. Free radical scavenging activities (1,1-diphenyl-2- picrylhydrazyl) size of, in the order of aglycone fraction > ethyl acetate fraction > 50% ethanol extract, aglycone fraction (45.10μg/mL) showed the highest radical scavenging activity. Reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, OSC50) on ROS generated in Fe3+-EDTA/H2O2 system was also, in the order of ethyl acetate fraction> aglycone fraction> 50% ethyl acetate fraction, ethyl acetate fraction (2.68μg/mL) was shown a great antioxidant capacity. The total antioxidant capacity of the ethyl acetate fraction was found to be greater than L-ascorbic acid, known as a typical hydrophilic antioxidant (6.88 μg/mL). The cellular protective effects of R. acetosa L. extracts on the 1O2-induced cellular damage of human erythrocytes were exhibited at all concentration-dependent (1∼25μg/mL). Especially, aglycone fraction (τ50, 104.80 min) in 25μg/mL showed the most protective effect among extracts. Components of the ethyl acetate fraction obtained from R. acetosa L. extracts were analyzed by TLC, HPLC chromatogram, LC/ESI-MS/MS. As a result, the ethyl acetate fraction contained several flavonoids, such as orientin, isoorientin, vitexin, isovitexin. These results indicate that the R. acetosa L. extracts can be used as antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging 1O2 and other ROS. Thus, the extracts of R. acetosa L. could be applicable to new anti-aging cosmeceutical ingredients.
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KEYWORD
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R, acetosa L, , antioxidative activity, photoaging, component analysis, cosmeceutical
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